{"id":4856,"date":"2025-08-14T10:18:28","date_gmt":"2025-08-14T10:18:28","guid":{"rendered":"https:\/\/molsentech.iapplabz.co.in\/blog\/pcr-%e8%88%87-qpcr-%e8%88%87-rt-pcr-%e8%88%87-rt-qpcr%ef%bc%9a%e6%9c%89%e4%bb%80%e9%ba%bc%e5%8d%80%e5%88%a5\/"},"modified":"2025-09-04T06:03:25","modified_gmt":"2025-09-04T06:03:25","slug":"pcr-%e8%88%87-qpcr-%e8%88%87-rt-pcr-%e8%88%87-rt-qpcr%ef%bc%9a%e6%9c%89%e4%bb%80%e9%ba%bc%e5%8d%80%e5%88%a5","status":"publish","type":"blog_post","link":"https:\/\/molsentech.iapplabz.co.in\/zh-hant\/post\/pcr-%e8%88%87-qpcr-%e8%88%87-rt-pcr-%e8%88%87-rt-qpcr%ef%bc%9a%e6%9c%89%e4%bb%80%e9%ba%bc%e5%8d%80%e5%88%a5","title":{"rendered":"PCR vs qPCR vs RT-PCR vs RT-qPCR\uff1a\u6709\u4ec0\u9ebc\u4e0d\u540c?"},"content":{"rendered":"\n

\u805a\u5408\u9176\u93c8\u5f0f\u53cd\u61c9\uff08Polymerase Chain Reaction, PCR\uff09\u662f\u4e00\u7a2e\u57fa\u790e\u7684\u5be6\u9a57\u5ba4\u6280\u8853\uff0c\u7528\u65bc\u64f4\u589e DNA \u5e8f\u5217\uff0c\u4f7f\u7814\u7a76\u4eba\u54e1\u80fd\u66f4\u5bb9\u6613\u5206\u6790\u7279\u5b9a\u57fa\u56e0\u6216\u95dc\u6ce8\u7684\u7247\u6bb5\u3002\u96a8\u8457\u6642\u9593\u767c\u5c55\uff0cPCR \u884d\u751f\u51fa\u591a\u7a2e\u8b8a\u5316\u7248\u672c\uff0c\u4ee5\u6eff\u8db3\u4e0d\u540c\u7684\u7814\u7a76\u8207\u8a3a\u65b7\u9700\u6c42\u3002\u4ee5\u4e0b\u5c07\u7c21\u55ae\u4ecb\u7d39 PCR\u3001qPCR\u3001RT-PCR\u3001RT-qPCR \u8207 dPCR\uff0c\u5e6b\u52a9\u60a8\u5728\u4e0d\u540c\u61c9\u7528\u4e2d\u9078\u64c7\u5408\u9069\u7684\u65b9\u6cd5\u3002<\/p>\n\n\n\n

\u4ec0\u9ebc\u662f PCR\uff08\u805a\u5408\u9176\u93c8\u5f0f\u53cd\u61c9\uff09\uff1f<\/strong><\/h3>\n\n\n\n

PCR\uff08\u53c8\u7a31\u70ba\u7d42\u9ede PCR\u3001\u50b3\u7d71 PCR \u6216\u5e38\u898f PCR\uff09\u662f\u4e00\u7a2e\u5ee3\u6cdb\u4f7f\u7528\u7684\u5be6\u9a57\u5ba4\u6280\u8853\uff0c\u80fd\u5c07\u7279\u5b9a DNA \u5e8f\u5217\u5927\u91cf\u64f4\u589e\uff0c\u5f9e\u6975\u5c11\u91cf\u7684\u8d77\u59cb\u6a23\u672c\u4e2d\u7522\u751f\u6578\u767e\u842c\u4efd\u62f7\u8c9d\u3002\u9019\u500b\u904e\u7a0b\u5305\u542b\u4e09\u500b\u4e3b\u8981\u6b65\u9a5f\uff1a<\/p>\n\n\n\n

    \n
  1. \u8b8a\u6027\uff08Denaturation\uff09<\/strong>\uff1a\u5c07\u53cd\u61c9\u6df7\u5408\u7269\u52a0\u71b1\u81f3\u7d04 94\u201398\u00b0C\uff0c\u4f7f\u96d9\u80a1 DNA \u5206\u958b\u6210\u70ba\u5169\u689d\u55ae\u80a1\u3002
    <\/li>\n\n\n\n
  2. \u9000\u706b\uff08Annealing\uff09<\/strong>\uff1a\u5c07\u6eab\u5ea6\u964d\u81f3\u7d04 50\u201365\u00b0C\uff0c\u4f7f\u77ed\u7247\u6bb5\u7684 DNA \u5f15\u5b50\uff08primer\uff09\u80fd\u8207\u55ae\u80a1 DNA \u6a21\u677f\u4e0a\u7684\u4e92\u88dc\u5e8f\u5217\u7d50\u5408\u3002
    <\/li>\n\n\n\n
  3. \u5ef6\u4f38\uff08Extension\uff09<\/strong>\uff1a\u5c07\u6eab\u5ea6\u5347\u81f3\u7d04 72\u00b0C\uff0c\u9019\u662f DNA \u805a\u5408\u9176\u7684\u6700\u4f73\u4f5c\u7528\u6eab\u5ea6\uff0c\u53ef\u5728\u5f15\u5b50\u4e0a\u5ef6\u4f38\u4e26\u6dfb\u52a0\u6838\u82f7\u9178\uff0c\u5408\u6210\u65b0\u7684 DNA \u93c8\u3002
    <\/li>\n<\/ol>\n\n\n\n

    \u9019\u4e09\u500b\u6b65\u9a5f\u6703\u5728\u4e00\u7a2e\u7a31\u70ba \u71b1\u5faa\u74b0\u5100\uff08thermal cycler\uff09<\/strong> \u7684\u8a2d\u5099\u4e2d\u91cd\u8907 20\u201340 \u500b\u5faa\u74b0\uff0c\u6700\u7d42\u4f7f\u76ee\u6a19 DNA \u5e8f\u5217\u5448\u6307\u6578\u500d\u6578\u589e\u6b96\u3002

    <\/p>\n\n\n\n

    \"PCR<\/figure>\n\n\n\n

    PCR \u6280\u8853\u7531 Kary Mullis<\/strong> \u65bc 1983 \u5e74\u767c\u660e\uff0c\u5fb9\u5e95\u9769\u65b0\u4e86\u5206\u5b50\u751f\u7269\u5b78\uff0c\u4f7f\u79d1\u5b78\u5bb6\u80fd\u5920\u5728\u4e0d\u9700\u8981\u5927\u91cf\u8d77\u59cb\u6a23\u672c\u7684\u60c5\u6cc1\u4e0b\uff0c\u7814\u7a76\u7279\u5b9a\u7684 DNA \u5340\u6bb5\u3002<\/p>\n\n\n\n

    PCR \u7684\u57fa\u672c\u7d44\u6210\u5305\u62ec\uff1a<\/p>\n\n\n\n

      \n
    • \u71b1\u5faa\u74b0\u5100\uff08thermal cycler\uff09<\/strong>\uff1a\u7cbe\u6e96\u63a7\u5236\u6eab\u5ea6\u8b8a\u5316
      <\/li>\n\n\n\n
    • DNA \u5f15\u5b50\uff08primers\uff09<\/strong>
      <\/strong><\/li>\n\n\n\n
    • \u6838\u82f7\u9178\uff08nucleotides\uff09<\/strong>
      <\/strong><\/li>\n\n\n\n
    • DNA \u805a\u5408\u9176\uff08\u6700\u5e38\u7528\u70ba Taq polymerase\uff09<\/strong>
      <\/strong><\/li>\n\n\n\n
    • \u5f85\u64f4\u589e\u7684 DNA \u6a21\u677f\uff08template\uff09<\/strong>
      <\/strong><\/li>\n<\/ul>\n\n\n\n

      \u9019\u9805\u65b9\u6cd5\u662f\u773e\u591a\u9818\u57df\u7684\u57fa\u790e\u5de5\u5177\uff0c\u5305\u62ec\u907a\u50b3\u5b78\u7814\u7a76\u3001\u6cd5\u91ab\u79d1\u5b78\u8207\u81e8\u5e8a\u8a3a\u65b7\uff0c\u4f7f\u5f97\u57fa\u56e0\u8907\u88fd\u3001\u75c5\u539f\u9ad4\u6aa2\u6e2c\u8207\u57fa\u56e0\u7a81\u8b8a\u5206\u6790\u7b49\u4efb\u52d9\u8b8a\u5f97\u66f4\u52a0\u53ef\u884c\u3002<\/p>\n\n\n\n

      \u71b1\u555f\u52d5 PCR\uff08Hot Start PCR\uff09<\/strong><\/h3>\n\n\n\n

      \u71b1\u555f\u52d5 PCR \u662f\u50b3\u7d71 PCR \u7684\u6539\u826f\u7248\u672c\uff0c\u65e8\u5728\u63d0\u5347\u7279\u7570\u6027\u8207\u7522\u91cf\u3002<\/p>\n\n\n\n

      \u5728\u50b3\u7d71 PCR \u4e2d\uff0cDNA \u805a\u5408\u9176\u5728\u53cd\u61c9\u6e96\u5099\u968e\u6bb5\u5373\u53ef\u80fd\u65bc\u8f03\u4f4e\u6eab\u4e0b\u88ab\u6d3b\u5316\uff0c\u5c0e\u81f4\u975e\u7279\u7570\u6027\u64f4\u589e\u4ee5\u53ca\u5f15\u5b50\u4e8c\u805a\u9ad4\uff08primer dimers\uff09\u7684\u5f62\u6210\u3002\u71b1\u555f\u52d5 PCR \u900f\u904e\u5728\u521d\u59cb\u8b8a\u6027\u6b65\u9a5f\u4e4b\u524d\u4fdd\u6301 DNA \u805a\u5408\u9176\u8655\u65bc\u4e0d\u6d3b\u5316\u72c0\u614b\uff0c\u4f86\u89e3\u6c7a\u9019\u500b\u554f\u984c\u3002\u5e38\u898b\u65b9\u6cd5\u662f\u5229\u7528\u5316\u5b78\u4fee\u98fe\u9176\u6216\u6297\u9ad4\uff0c\u5728\u5ba4\u6eab\u4e0b\u66ab\u6642\u6291\u5236\u805a\u5408\u9176\u7684\u6d3b\u6027\u3002<\/p>\n\n\n\n

      \u7576\u53cd\u61c9\u6df7\u5408\u7269\u5728\u8b8a\u6027\u968e\u6bb5\u52a0\u71b1\u5f8c\uff0c\u9019\u4e9b\u4fee\u98fe\u4fbf\u6703\u88ab\u79fb\u9664\uff0c\u805a\u5408\u9176\u96a8\u4e4b\u88ab\u6d3b\u5316\u3002\u9019\u7a2e\u53d7\u63a7\u7684\u6d3b\u5316\u65b9\u5f0f\u80fd\u907f\u514d\u4e0d\u5fc5\u8981\u7684\u64f4\u589e\uff0c\u6700\u7d42\u7522\u751f\u66f4\u4e7e\u6de8\u4e14\u66f4\u7cbe\u78ba\u7684 PCR \u7d50\u679c\u3002<\/p>\n\n\n\n

      \u4ec0\u9ebc\u662f qPCR\uff08\u5b9a\u91cf PCR\uff09\/ \u5373\u6642 PCR\uff08Real-time PCR\uff09\uff1f<\/strong><\/h3>\n\n\n\n

      \u5b9a\u91cf PCR\uff0c\u901a\u5e38\u7a31\u70ba qPCR \u6216\u5373\u6642 PCR\uff0c\u662f\u4e00\u7a2e\u80fd\u540c\u6642 \u64f4\u589e\u4e26\u5b9a\u91cf DNA \u5e8f\u5217<\/strong> \u7684\u5be6\u9a57\u5ba4\u6280\u8853\u3002<\/p>\n\n\n\n

      \u4e0d\u540c\u65bc\u50b3\u7d71 PCR \u50c5\u80fd\u5728\u53cd\u61c9\u7d50\u675f\u6642\u986f\u793a DNA \u7684\u6709\u7121\uff0cqPCR \u53ef\u5728 \u64f4\u589e\u904e\u7a0b\u4e2d\u6e2c\u91cf DNA \u6fc3\u5ea6<\/strong>\u3002\u5176\u539f\u7406\u662f\u4f7f\u7528\u87a2\u5149\u67d3\u5291\u6216\u63a2\u91dd\uff0c\u7576\u5176\u8207\u96d9\u80a1 DNA \u7d50\u5408\u6642\u6703\u767c\u51fa\u87a2\u5149\u3002\u96a8\u8457 DNA \u7684\u64f4\u589e\uff0c\u87a2\u5149\u5f37\u5ea6\u6703\u6210\u6bd4\u4f8b\u589e\u52a0\uff0c\u9032\u800c\u5be6\u73fe\u5373\u6642\u76e3\u6e2c\u3002<\/p>\n\n\n\n

      \u9019\u7a2e\u65b9\u6cd5\u8b93\u79d1\u5b78\u5bb6\u80fd\u5728\u6bcf\u500b\u5faa\u74b0\u4e2d\u6e2c\u91cf DNA \u7684\u6578\u91cf\uff0c\u63d0\u4f9b \u8cea\u6027\u8207\u91cf\u5316\u6578\u64da<\/strong>\u3002qPCR \u5177\u5099\u6975\u9ad8\u9748\u654f\u5ea6\u8207\u7279\u7570\u6027\uff0c\u56e0\u6b64\u5ee3\u6cdb\u61c9\u7528\u65bc \u57fa\u56e0\u8868\u73fe\u5206\u6790\u3001\u75c5\u539f\u9ad4\u6aa2\u6e2c\u8207\u57fa\u56e0\u8b8a\u7570\u7814\u7a76<\/strong>\u3002\u7531\u65bc\u80fd\u5feb\u901f\u4e14\u7cbe\u78ba\u5730\u5b9a\u91cf DNA\uff0cqPCR \u5df2\u6210\u70ba\u5206\u5b50\u751f\u7269\u5b78\u8207\u91ab\u5b78\u8a3a\u65b7\u4e2d\u4e0d\u53ef\u6216\u7f3a\u7684\u6280\u8853\u3002<\/p>\n\n\n\n

      \u4ec0\u9ebc\u662f RT-PCR\uff08\u53cd\u8f49\u9304 PCR\uff09\uff1f<\/strong><\/h3>\n\n\n\n

      RT-PCR\uff08Reverse Transcription PCR\uff0c\u53cd\u8f49\u9304\u805a\u5408\u9176\u93c8\u5f0f\u53cd\u61c9\uff09\u662f\u4e00\u7a2e\u80fd\u8b93\u79d1\u5b78\u5bb6\u7814\u7a76 RNA \u7684\u6280\u8853\uff0c\u65b9\u6cd5\u662f \u5c07 RNA \u8f49\u63db\u70ba DNA<\/strong>\u3002<\/p>\n\n\n\n

      \u9019\u500b\u904e\u7a0b\u7531\u4e00\u7a2e\u540d\u70ba \u53cd\u8f49\u9304\u9176\uff08reverse transcriptase\uff09<\/strong> \u7684\u9175\u7d20\u958b\u59cb\uff0c\u5c07 RNA \u8f49\u63db\u70ba\u4e92\u88dc DNA\uff08cDNA\uff09\u3002\u63a5\u8457\uff0ccDNA \u7d93\u7531 PCR \u64f4\u589e\uff0c\u4fbf\u80fd\u9032\u884c\u66f4\u6df1\u5165\u7684\u5206\u6790\u3002<\/p>\n\n\n\n

      RT-PCR \u5c0d\u65bc \u6aa2\u6e2c RNA \u75c5\u6bd2<\/strong> \u53ca \u7814\u7a76\u57fa\u56e0\u8868\u73fe<\/strong> \u7279\u5225\u6709\u50f9\u503c\u3002\u7531\u65bc\u80fd\u64f4\u589e\u7279\u5b9a RNA \u5e8f\u5217\uff0c\u5b83\u5728\u7814\u7a76\u8207\u8a3a\u65b7\u4e2d\u90fd\u662f\u5f37\u5927\u7684\u5de5\u5177\u3002\u900f\u904e\u63ed\u793a\u57fa\u56e0\u6d3b\u6027\u8207 RNA \u578b\u751f\u7269\uff08\u5982\u75c5\u6bd2\uff09\u7684\u5b58\u5728\uff0cRT-PCR \u5df2\u6210\u70ba\u5206\u5b50\u751f\u7269\u5b78\u4e2d\u7684\u6838\u5fc3\u65b9\u6cd5\u4e4b\u4e00\u3002<\/p>\n\n\n\n

      \u4ec0\u9ebc\u662f RT-qPCR\uff08\u53cd\u8f49\u9304\u5b9a\u91cf PCR\uff09\uff1f<\/strong><\/h3>\n\n\n\n

      RT-qPCR\uff08Reverse Transcription Quantitative PCR\uff0c\u53cd\u8f49\u9304\u5b9a\u91cf\u805a\u5408\u9176\u93c8\u5f0f\u53cd\u61c9\uff09\u662f\u4e00\u7a2e\u5f37\u5927\u6280\u8853\uff0c\u7d50\u5408\u4e86 RNA \u7684\u53cd\u8f49\u9304\u8207\u5b9a\u91cf PCR<\/strong>\u3002\u9700\u6ce8\u610f\uff0c\u9019\u88e1\u7684\u300cRT\u300d\u4ee3\u8868 Reverse Transcription\uff08\u53cd\u8f49\u9304\uff09<\/strong>\uff0c\u800c\u4e0d\u662f Real-Time\uff08\u5373\u6642\uff09<\/strong>\u3002<\/p>\n\n\n\n

      \u6b64\u65b9\u6cd5\u80fd\u540c\u6642 \u6aa2\u6e2c\u8207\u5b9a\u91cf RNA \u6a19\u7684\u7269<\/strong>\uff0c\u56e0\u6b64\u5728 \u57fa\u56e0\u8868\u73fe\u5206\u6790\u8207 RNA \u75c5\u6bd2\u6aa2\u6e2c<\/strong> \u4e2d\u4e0d\u53ef\u6216\u7f3a\u3002\u6d41\u7a0b\u70ba\uff1a\u9996\u5148\u4f7f\u7528\u53cd\u8f49\u9304\u9176\u5c07 RNA \u8f49\u63db\u70ba cDNA\uff0c\u63a5\u8457\u4ee5 cDNA \u4f5c\u70ba\u6a21\u677f\u9032\u884c\u5b9a\u91cf PCR\uff0c\u900f\u904e\u87a2\u5149\u5075\u6e2c\u5728\u5373\u6642\u53cd\u61c9\u4e2d\u64f4\u589e\u4e26\u6e2c\u91cf\u76ee\u6a19\u5e8f\u5217\u7684\u6578\u91cf\u3002<\/p>\n\n\n\n

      RT-qPCR \u5177\u6709\u9ad8\u5ea6\u9748\u654f\u6027\u8207\u7279\u7570\u6027\uff0c\u80fd\u5075\u6e2c\u4f4e\u542b\u91cf RNA \u5206\u5b50\u3002\u5176\u5b9a\u91cf\u7279\u6027\u53ef\u7cbe\u6e96\u6e2c\u91cf\u57fa\u56e0\u8868\u73fe\u6c34\u5e73\uff0c\u70ba\u7d30\u80de\u529f\u80fd\u8207\u75be\u75c5\u6a5f\u5236\u63d0\u4f9b\u91cd\u8981\u6d1e\u5bdf\u3002\u540c\u6642\uff0c\u5b83\u4e5f\u662f RNA \u75c5\u6bd2\u6aa2\u6e2c\u7684\u6a19\u6e96\u65b9\u6cd5<\/strong>\uff0c\u4f8b\u5982 \u65b0\u51a0\u75c5\u6bd2\uff08SARS-CoV-2\uff09<\/strong>\uff0c\u56e0\u6b64\u5728\u75be\u75c5\u8a3a\u65b7\u8207\u7ba1\u7406\u4e2d\u767c\u63ee\u4e86\u5de8\u5927\u4f5c\u7528\u3002<\/p>\n\n\n\n

      \u4ec0\u9ebc\u662f dPCR\uff08\u6578\u4f4d PCR\uff09\uff1f<\/strong><\/h3>\n\n\n\n

      \u6578\u4f4d PCR\uff08Digital PCR, dPCR\uff09\u662f\u4e00\u7a2e\u5148\u9032\u7684 DNA \u8207 RNA \u5075\u6e2c\u8207\u5b9a\u91cf\u65b9\u6cd5\u3002
      <\/p>\n\n\n\n

      \"PCR<\/figure>\n\n\n\n

      \u4e00\u500b dPCR \u7684\u5de5\u4f5c\u6d41\u7a0b\u53ef\u5206\u70ba\u56db\u500b\u968e\u6bb5\uff1a\u6e96\u5099\uff08Preparation\uff09\u3001\u5206\u9694\uff08Partitioning\uff09\u3001\u64f4\u589e\uff08Amplification\uff09\u3001\u5206\u6790\uff08Analysis\uff09<\/strong>\u3002<\/p>\n\n\n\n

        \n
      1. \u6e96\u5099\uff08Preparation\uff09<\/strong>
        <\/strong> \u9996\u5148\uff0c\u5f9e\u6a23\u672c\u4e2d\u63d0\u53d6 DNA \u6216 RNA\uff0c\u4e26\u9032\u884c\u7d14\u5316\uff0c\u518d\u8207\u53cd\u61c9\u6210\u5206\u6df7\u5408\uff0c\u9019\u4e9b\u6210\u5206\u5305\u62ec\u5f15\u5b50\uff08primers\uff09\u3001\u6838\u82f7\u9178\uff08nucleotides\uff09\u3001DNA \u805a\u5408\u9176\u4ee5\u53ca\u87a2\u5149\u63a2\u91dd\u6216\u67d3\u5291\u3002
        <\/li>\n\n\n\n
      2. \u5206\u9694\uff08Partitioning\uff09<\/strong>
        <\/strong> \u63a5\u8457\uff0c\u5c07\u6df7\u5408\u7269\u5206\u9694\u6210\u6578\u5343\u500b\u5fae\u5c0f\u3001\u7368\u7acb\u7684\u53cd\u61c9\u5340\u57df\uff0c\u5e38\u7528\u7684\u6280\u8853\u5305\u62ec\u5fae\u6d41\u9ad4\u6676\u7247\u6216\u6db2\u6ef4\u7522\u751f\u5668\u3002\u6bcf\u500b\u5206\u9694\u4e2d\u53ef\u80fd\u542b\u6709\u96f6\u500b\u3001\u4e00\u500b\u6216\u5c11\u6578\u5e7e\u500b\u76ee\u6a19 DNA \u5206\u5b50\u3002\u9019\u7a2e\u7368\u7279\u7684\u65b9\u6cd5\u80fd\u4ee5\u6975\u9ad8\u7684\u7cbe\u6e96\u5ea6\u9396\u5b9a\u4e26\u8a08\u7b97\u76ee\u6a19\u5206\u5b50\u3002
        <\/li>\n\n\n\n
      3. \u64f4\u589e\uff08Amplification\uff09<\/strong>
        <\/strong> \u5728\u64f4\u589e\u968e\u6bb5\uff0c\u6bcf\u500b\u5206\u9694\u6703\u9032\u884c\u71b1\u5faa\u74b0\uff08\u8207\u50b3\u7d71 PCR \u76f8\u4f3c\uff09\uff0c\u4ee5\u64f4\u589e\u5176\u4e2d\u7684\u76ee\u6a19 DNA\u3002\u82e5\u76ee\u6a19 DNA \u88ab\u64f4\u589e\u6210\u529f\uff0c\u8a72\u5206\u9694\u6703\u7522\u751f\u87a2\u5149\u8a0a\u865f\u3002
        <\/li>\n\n\n\n
      4. \u5206\u6790\uff08Analysis\uff09<\/strong>
        <\/strong> \u6700\u5f8c\uff0c\u7d71\u8a08\u6240\u6709\u300c\u967d\u6027\u5206\u9694\u300d\uff08\u7522\u751f\u87a2\u5149\u7684\u5206\u9694\uff09\u6578\u91cf\uff0c\u4e26\u5229\u7528\u7d71\u8a08\u5b78\u65b9\u6cd5\u8a08\u7b97\u51fa\u539f\u59cb\u6a23\u672c\u4e2d DNA \u6216 RNA \u7684\u7d55\u5c0d\u6578\u91cf\u3002
        <\/li>\n<\/ol>\n\n\n\n

        dPCR \u7684\u6700\u5927\u7279\u8272<\/strong>\u4e4b\u4e00\u662f\u80fd\u5920\u63d0\u4f9b \u7d55\u5c0d\u5b9a\u91cf\uff08Absolute Quantification\uff09<\/strong>\u3002\u63db\u53e5\u8a71\u8aaa\uff0c\u5b83\u80fd\u76f4\u63a5\u6e2c\u91cf DNA \u6216 RNA \u5206\u5b50\u7684\u78ba\u5207\u6578\u91cf\uff0c\u800c\u4e0d\u9700\u8981\u4f9d\u8cf4\u50cf qPCR \u90a3\u6a23\u7684\u6a19\u6e96\u66f2\u7dda\u6bd4\u8f03\u3002<\/p>\n\n\n\n

        \u9019\u4f7f dPCR \u7279\u5225\u9069\u5408\u7528\u65bc\u9700\u8981\u6975\u9ad8\u7cbe\u6e96\u5ea6\u7684\u60c5\u5883\u3002\u540c\u6642\uff0c\u5b83\u5c0d\u6a23\u672c\u4e2d\u53ef\u80fd\u5e72\u64fe\u7d50\u679c\u7684\u96dc\u8cea\u4e0d\u654f\u611f\uff0c\u5373\u4f7f\u5728\u8907\u96dc\u6a23\u672c\uff08\u5982\u8840\u6db2\u6216\u74b0\u5883\u6a23\u672c\uff09\u4e2d\uff0c\u4f9d\u7136\u80fd\u4fdd\u6301\u9ad8\u5ea6\u53ef\u9760\u8207\u4e00\u81f4\u6027\u3002<\/p>\n\n\n\n

        \u4e0d\u904e\uff0cdPCR \u4e5f\u6709\u5176\u9650\u5236\uff1a\u9700\u8981\u5c08\u9580\u7684\u8a2d\u5099\u4f86\u7522\u751f\u9019\u4e9b\u5fae\u5c0f\u5206\u9694\u4e26\u9032\u884c\u5075\u6e2c\uff0c\u64cd\u4f5c\u76f8\u5c0d\u8907\u96dc\u3002\u4f46\u5728\u8ffd\u6c42 \u9ad8\u9748\u654f\u5ea6\u3001\u7cbe\u78ba\u6027\u8207\u53ef\u9760\u6027<\/strong> \u7684\u61c9\u7528\u4e0a\uff0cdPCR \u5e7e\u4e4e\u7121\u53ef\u53d6\u4ee3\u3002<\/p>\n\n\n\n

        PCR vs qPCR vs RT-PCR vs RT-qPCR vs dPCR\uff1a\u5dee\u7570\u6bd4\u8f03<\/strong><\/h2>\n\n\n\n

        \u7406\u89e3 PCR\u3001qPCR\u3001RT-PCR\u3001RT-qPCR \u8207 dPCR \u4e4b\u9593\u7684\u7d30\u5fae\u5dee\u7570\uff0c\u5c0d\u65bc\u91dd\u5c0d\u7279\u5b9a\u7814\u7a76\u8207\u8a3a\u65b7\u9700\u6c42\u9078\u64c7\u5408\u9069\u7684\u5206\u5b50\u751f\u7269\u5b78\u6280\u8853\u81f3\u95dc\u91cd\u8981\u3002\u6bcf\u7a2e\u65b9\u6cd5\u90fd\u6709\u5176\u7368\u7279\u7684\u512a\u52e2\u8207\u61c9\u7528\u3002\u4ee5\u4e0b\u9010\u9ede\u8aaa\u660e\uff1a<\/p>\n\n\n\n

        \u8d77\u59cb\u6750\u6599\uff08Starting Material\uff09<\/strong><\/h3>\n\n\n\n
          \n
        • PCR \u8207 qPCR<\/strong>\uff1a\u4f7f\u7528 DNA \u4f5c\u70ba\u8d77\u59cb\u6750\u6599\uff0c\u9069\u5408\u7528\u65bc\u7279\u5b9a DNA \u5e8f\u5217\u7684\u64f4\u589e\u8207\u5b9a\u91cf\u3002
          <\/li>\n\n\n\n
        • RT-PCR \u8207 RT-qPCR<\/strong>\uff1a\u4ee5 RNA \u70ba\u8d77\u59cb\u6750\u6599\uff0c\u9700\u5148\u900f\u904e\u53cd\u8f49\u9304\u8f49\u63db\u70ba cDNA\uff0c\u518d\u9032\u884c\u5206\u6790\uff0c\u56e0\u6b64\u7279\u5225\u9069\u5408\u7814\u7a76 RNA \u75c5\u6bd2\u8207\u57fa\u56e0\u8868\u73fe\u3002
          <\/li>\n\n\n\n
        • dPCR<\/strong>\uff1a\u5177\u5099\u5f48\u6027\uff0c\u53ef\u4f7f\u7528 DNA\uff0c\u6216\u5728\u53cd\u8f49\u9304\u5f8c\u4f7f\u7528 RNA\u3002\u5176\u7279\u8272\u662f\u80fd\u9032\u884c \u7d55\u5c0d\u5b9a\u91cf<\/strong>\uff0c\u7121\u8ad6\u8d77\u59cb\u6750\u6599\u70ba\u4f55\uff0c\u90fd\u80fd\u63d0\u4f9b\u7121\u8207\u502b\u6bd4\u7684\u7cbe\u6e96\u5ea6\u3002
          <\/li>\n<\/ul>\n\n\n\n

          \u5b9a\u91cf\u80fd\u529b\uff08Quantification\uff09<\/strong><\/h3>\n\n\n\n
            \n
          • PCR\uff08\u50b3\u7d71 PCR\uff09<\/strong>\uff1a\u50c5\u80fd\u6aa2\u6e2c DNA \u662f\u5426\u5b58\u5728\uff0c\u7121\u6cd5\u9032\u884c\u5b9a\u91cf\u3002
            <\/li>\n\n\n\n
          • qPCR<\/strong>\uff1a\u5728\u64f4\u589e\u904e\u7a0b\u4e2d\u5373\u6642\u8ffd\u8e64 DNA\uff0c\u80fd\u9032\u884c\u76f8\u5c0d\u5b9a\u91cf\u3002
            <\/li>\n\n\n\n
          • RT-PCR<\/strong>\uff1a\u53ef\u6aa2\u6e2c RNA\uff0c\u4f46\u540c\u6a23\u7121\u6cd5\u91cf\u5316\u3002
            <\/li>\n\n\n\n
          • RT-qPCR<\/strong>\uff1a\u7d50\u5408 RNA \u5206\u6790\u8207\u5373\u6642\u5b9a\u91cf\uff0c\u53ef\u7cbe\u6e96\u6e2c\u91cf\u57fa\u56e0\u8868\u73fe\u6216\u75c5\u6bd2\u91cf\u3002
            <\/li>\n\n\n\n
          • dPCR<\/strong>\uff1a\u63d0\u4f9b \u7d55\u5c0d\u5b9a\u91cf<\/strong>\uff0c\u80fd\u76f4\u63a5\u8a08\u7b97\u6838\u9178\u5206\u5b50\u6578\u76ee\uff0c\u7121\u9700\u6a19\u6e96\u66f2\u7dda\uff0c\u7cbe\u6e96\u5ea6\u6700\u9ad8\u3002<\/li>\n<\/ul>\n\n\n\n

            \u9748\u654f\u5ea6\uff08Sensitivity\uff09<\/strong><\/h3>\n\n\n\n
              \n
            • PCR<\/strong>\uff1a\u9748\u654f\u5ea6\u4e2d\u7b49\uff0c\u8db3\u4ee5\u61c9\u4ed8\u8a31\u591a\u7814\u7a76\u9700\u6c42\uff0c\u4f46\u5c0d\u65bc\u7a00\u6709\u6216\u4f4e\u6fc3\u5ea6\u76ee\u6a19\u6548\u679c\u6709\u9650\u3002
              <\/li>\n\n\n\n
            • qPCR<\/strong>\uff1a\u9748\u654f\u5ea6\u9ad8\uff0c\u53ef\u5373\u6642\u5075\u6e2c\u5c11\u91cf DNA\u3002
              <\/li>\n\n\n\n
            • RT-PCR<\/strong>\uff1a\u5c0d RNA \u6aa2\u6e2c\u6709\u4e2d\u7b49\u9748\u654f\u5ea6\uff0c\u4f46\u5b9a\u91cf\u7cbe\u78ba\u5ea6\u4e0d\u8db3\u3002
              <\/li>\n\n\n\n
            • RT-qPCR<\/strong>\uff1a\u7d50\u5408\u9ad8\u9748\u654f\u5ea6\u8207\u5b9a\u91cf\u80fd\u529b\uff0c\u9069\u5408\u5206\u6790\u4f4e\u542b\u91cf RNA\u3002
              <\/li>\n\n\n\n
            • dPCR<\/strong>\uff1a\u9748\u654f\u5ea6\u6700\u9ad8\uff0c\u53ef\u5075\u6e2c\u6975\u4f4e\u6fc3\u5ea6\u76ee\u6a19\u8207\u4f4e\u983b\u7a81\u8b8a\uff0c\u5c0d\u9700\u8981\u6975\u9ad8\u7cbe\u78ba\u5ea6\u7684\u61c9\u7528\u4e0d\u53ef\u6216\u7f3a\u3002<\/li>\n<\/ul>\n\n\n\n

              \u7cbe\u78ba\u5ea6\uff08Precision\uff09<\/strong><\/h3>\n\n\n\n

              \u7cbe\u78ba\u5ea6\u6307\u7684\u662f PCR \u6280\u8853\u80fd\u5426 \u6301\u7e8c\u63d0\u4f9b\u6e96\u78ba\u4e14\u53ef\u91cd\u73fe\u7684\u6578\u64da<\/strong>\u3002\u5c0d\u65bc\u9700\u8981\u7d30\u7dfb\u5b9a\u91cf\u7684\u5be6\u9a57\uff08\u5982\u5fae\u5c0f\u57fa\u56e0\u8868\u73fe\u5dee\u7570\u6216\u4f4e\u983b\u7a81\u8b8a\u6aa2\u6e2c\uff09\uff0c\u7cbe\u78ba\u5ea6\u81f3\u95dc\u91cd\u8981\u3002<\/p>\n\n\n\n

                \n
              • \u50b3\u7d71 PCR<\/strong>\uff1a\u9069\u5408\u6838\u9178\u64f4\u589e\uff0c\u4f46\u5728\u5b9a\u91cf\u7cbe\u78ba\u5ea6\u4e0a\u4e0d\u8db3\u3002
                <\/li>\n\n\n\n
              • qPCR<\/strong>\uff1a\u900f\u904e\u5373\u6642\u5b9a\u91cf\u6578\u64da\u63d0\u5347\u7cbe\u78ba\u5ea6\uff0c\u4f46\u7d50\u679c\u4ecd\u53ef\u80fd\u4f9d\u8cf4\u6a19\u6e96\u66f2\u7dda\u3002
                <\/li>\n\n\n\n
              • RT-PCR<\/strong>\uff1a\u91cd\u9ede\u5728 RNA \u5075\u6e2c\uff0c\u5c6c\u65bc\u5b9a\u6027\u5206\u6790\uff0c\u5c0d\u7cbe\u78ba\u5ea6\u8981\u6c42\u4e0d\u9ad8\u3002
                <\/li>\n\n\n\n
              • RT-qPCR<\/strong>\uff1a\u7d50\u5408\u53cd\u8f49\u9304\u8207\u5373\u6642\u5b9a\u91cf\u5206\u6790\uff0c\u80fd\u63d0\u5347 RNA \u5b9a\u91cf\u7684\u6e96\u78ba\u6027\u3002
                <\/li>\n\n\n\n
              • dPCR<\/strong>\uff1a\u7cbe\u78ba\u5ea6\u6700\u9ad8\uff0c\u900f\u904e\u5c07\u6a23\u672c\u5206\u9694\u6210\u6578\u5343\u53cd\u61c9\u55ae\u5143\uff0c\u907f\u514d\u4f9d\u8cf4\u6a19\u6e96\u66f2\u7dda\u4e26\u964d\u4f4e\u8b8a\u7570\uff0c\u975e\u5e38\u9069\u5408\u7a00\u6709\u7a81\u8b8a\u6aa2\u6e2c\u8207\u7d55\u5c0d\u6838\u9178\u5b9a\u91cf\u3002<\/li>\n<\/ul>\n\n\n\n

                \u64cd\u4f5c\u96e3\u6613\u5ea6\uff08Ease of Use\uff09<\/strong><\/h3>\n\n\n\n

                \u64cd\u4f5c\u4fbf\u5229\u6027\u6703\u5f71\u97ff\u6280\u8853\u5728\u5be6\u9a57\u5ba4\u4e2d\u7684\u666e\u53ca\u8207\u6548\u7387\uff0c\u4e5f\u6d89\u53ca\u5de5\u4f5c\u6d41\u7a0b\u3001\u8a13\u7df4\u9700\u6c42\u8207\u61c9\u7528\u53ef\u884c\u6027\u3002<\/p>\n\n\n\n

                  \n
                • \u50b3\u7d71 PCR<\/strong>\uff1a\u6700\u7c21\u55ae\uff0c\u50c5\u9700\u57fa\u672c\u8a2d\u5099\u8207\u64cd\u4f5c\u6d41\u7a0b\uff0c\u591a\u6578\u5be6\u9a57\u5ba4\u7686\u80fd\u4f7f\u7528\u3002
                  <\/li>\n\n\n\n
                • qPCR<\/strong>\uff1a\u9700\u5373\u6642\u87a2\u5149\u76e3\u6e2c\uff0c\u5fc5\u9808\u4f7f\u7528\u5c08\u7528\u5100\u5668\u4e26\u5177\u5099\u4e00\u5b9a\u6280\u8853\u80fd\u529b\u3002
                  <\/li>\n\n\n\n
                • RT-PCR<\/strong>\uff1a\u589e\u52a0\u53cd\u8f49\u9304\u6b65\u9a5f\uff0c\u64cd\u4f5c\u6bd4\u50b3\u7d71 PCR \u8907\u96dc\u3002
                  <\/li>\n\n\n\n
                • RT-qPCR<\/strong>\uff1a\u9700\u540c\u6642\u9032\u884c\u53cd\u8f49\u9304\u8207\u5373\u6642\u76e3\u6e2c\uff0c\u8907\u96dc\u5ea6\u66f4\u9ad8\uff0c\u9700\u9032\u968e\u6280\u8853\u8207\u8a2d\u5099\u3002
                  <\/li>\n\n\n\n
                • dPCR<\/strong>\uff1a\u6700\u8907\u96dc\uff0c\u9700\u8981\u5c08\u9580\u7684\u5206\u9694\u5100\u5668\u8207\u6578\u64da\u5206\u6790\u5de5\u5177\uff0c\u96d6\u7136\u7cbe\u6e96\u5ea6\u7121\u53ef\u6bd4\u64ec\uff0c\u4f46\u64cd\u4f5c\u96e3\u5ea6\u6700\u5927\u3002<\/li>\n<\/ul>\n\n\n\n

                  \u6210\u672c\uff08Cost\uff09<\/strong><\/h3>\n\n\n\n

                  \u6210\u672c\u662f\u9078\u64c7 PCR \u6280\u8853\u7684\u91cd\u8981\u8003\u91cf\uff0c\u5c24\u5176\u5c0d\u65bc\u9810\u7b97\u6709\u9650\u7684\u5be6\u9a57\u5ba4\u3002<\/p>\n\n\n\n

                    \n
                  • \u50b3\u7d71 PCR<\/strong>\uff1a\u6700\u4fbf\u5b9c\uff0c\u53ea\u9700\u57fa\u672c\u8a66\u5291\u8207\u8a2d\u5099\u3002
                    <\/li>\n\n\n\n
                  • qPCR<\/strong>\uff1a\u6210\u672c\u8f03\u9ad8\uff0c\u9700\u8981\u87a2\u5149\u63a2\u91dd\u8207\u5373\u6642\u6aa2\u6e2c\u7cfb\u7d71\u3002
                    <\/li>\n\n\n\n
                  • RT-PCR<\/strong>\uff1a\u6bd4\u50b3\u7d71 PCR \u66f4\u6602\u8cb4\uff0c\u9700\u984d\u5916\u7684\u53cd\u8f49\u9304\u8a66\u5291\u3002
                    <\/li>\n\n\n\n
                  • RT-qPCR<\/strong>\uff1a\u7d50\u5408\u53cd\u8f49\u9304\u8207\u5373\u6642\u5b9a\u91cf\uff0c\u6210\u672c\u66f4\u9ad8\u3002
                    <\/li>\n\n\n\n
                  • dPCR<\/strong>\uff1a\u6700\u6602\u8cb4\uff0c\u9700\u8981\u9ad8\u7aef\u5206\u9694\u6280\u8853\u3001\u5c08\u7528\u5100\u5668\u8207\u8017\u6750\u3002<\/li>\n<\/ul>\n\n\n\n

                    \u5927\u91cf\u6a23\u672c\u8655\u7406\uff08Large Volume Tested Samples\uff09<\/strong><\/h3>\n\n\n\n

                    \u5728\u5927\u91cf\u6a23\u672c\u5206\u6790\u4e2d\uff0cPCR \u6280\u8853\u9700\u80fd\u8655\u7406\u8db3\u5920\u6a23\u672c\u91cf\u4ee5\u907f\u514d\u7a00\u6709\u76ee\u6a19\u907a\u6f0f\uff0c\u5426\u5247\u5b9a\u91cf\u6703\u4e0d\u6e96\u78ba\u3002<\/p>\n\n\n\n

                      \n
                    • \u50b3\u7d71 PCR \u8207 RT-PCR<\/strong>\uff1a\u80fd\u8655\u7406\u4e2d\u91cf\u6a23\u672c\uff0c\u4f46\u50c5\u9650\u5b9a\u6027\u5206\u6790\u3002
                      <\/li>\n\n\n\n
                    • qPCR \u8207 RT-qPCR<\/strong>\uff1a\u9069\u5408\u8f03\u5927\u91cf\u6a23\u672c\uff0c\u80fd\u5728\u6df7\u5408\u6a23\u672c\u4e2d\u5075\u6e2c\u4f4e\u6fc3\u5ea6\u6838\u9178\u3002
                      <\/li>\n\n\n\n
                    • dPCR<\/strong>\uff1a\u96d6\u7136\u7cbe\u78ba\u5ea6\u6700\u9ad8\uff0c\u4f46\u56e0\u5206\u9694\u7b56\u7565\u901a\u5e38\u8655\u7406\u9ad4\u7a4d\u8f03\u5c0f\uff0c\u4e0d\u592a\u9069\u5408\u5927\u6a23\u672c\u91cf\u61c9\u7528\u3002
                      <\/li>\n<\/ul>\n\n\n\n

                      \u61c9\u7528\uff08Uses\uff09<\/strong><\/h3>\n\n\n\n
                        \n
                      • \u50b3\u7d71 PCR<\/strong>\uff1a\u57fa\u56e0\u8907\u88fd\u3001\u7a81\u8b8a\u6aa2\u6e2c\u3001\u57fa\u56e0\u5206\u578b\u3002
                        <\/li>\n\n\n\n
                      • qPCR<\/strong>\uff1a\u57fa\u56e0\u8868\u73fe\u5b9a\u91cf\u3001\u75c5\u539f\u9ad4\u6aa2\u6e2c\u3001\u57fa\u56e0\u8b8a\u7570\u5206\u6790\u3002
                        <\/li>\n\n\n\n
                      • RT-PCR<\/strong>\uff1aRNA \u7814\u7a76\uff0c\u5982 RNA \u75c5\u6bd2\u6aa2\u6e2c\u8207\u57fa\u56e0\u8868\u73fe\u5206\u6790\u3002
                        <\/li>\n\n\n\n
                      • RT-qPCR<\/strong>\uff1a\u7d50\u5408 RNA \u5075\u6e2c\u8207\u7cbe\u78ba\u5b9a\u91cf\uff0c\u9069\u5408\u76e3\u6e2c\u75c5\u6bd2\u8f09\u91cf\u8207\u57fa\u56e0\u6d3b\u6027\u6e2c\u91cf\u3002
                        <\/li>\n\n\n\n
                      • dPCR<\/strong>\uff1a\u7d55\u5c0d\u7cbe\u6e96\u7684\u61c9\u7528\uff0c\u5982\u7a00\u6709\u7a81\u8b8a\u6aa2\u6e2c\u3001\u8907\u96dc\u6a23\u672c\u5206\u6790\u3001\u764c\u75c7\u6db2\u614b\u6d3b\u6aa2\u3002<\/li>\n<\/ul>\n\n\n\n
                        \u56e0\u7d20 Factor<\/strong><\/td>PCR<\/strong><\/td>qPCR<\/strong><\/td>RT-PCR<\/strong><\/td>RT-qPCR<\/strong><\/td>dPCR<\/strong><\/td><\/tr>
                        \u8d77\u59cb\u6750\u6599 Starting Material<\/strong><\/td>DNA<\/td>DNA<\/td>RNA\uff08\u8f49\u63db\u70ba cDNA\uff09<\/td>RNA\uff08\u8f49\u63db\u70ba cDNA\uff09<\/td>DNA \u6216 RNA\uff08\u7d93 cDNA\uff09<\/td><\/tr>
                        \u5b9a\u91cf\u80fd\u529b Quantification<\/strong><\/td>\u5426<\/td>\u662f\uff08\u5373\u6642\uff0c\u76f8\u5c0d\u5b9a\u91cf\uff09<\/td>\u5426<\/td>\u662f\uff08\u5373\u6642\uff0c\u76f8\u5c0d\u5b9a\u91cf\uff09<\/td>\u662f\uff08\u7d55\u5c0d\u5b9a\u91cf\uff0c\u7cbe\u78ba\u8a08\u6578\uff09<\/td><\/tr>
                        \u9748\u654f\u5ea6 Sensitivity<\/strong><\/td>\u4e2d\u7b49<\/td>\u9ad8<\/td>\u4e2d\u7b49<\/td>\u9ad8<\/td>\u6975\u9ad8<\/td><\/tr>
                        \u7cbe\u78ba\u5ea6 Precision<\/strong><\/td>\u53ef\u9760\uff08\u5224\u65b7\u6709\u7121\uff09<\/td>\u9ad8\uff08\u5373\u6642\u6578\u64da\uff09<\/td>\u53ef\u9760\uff08\u5b9a\u6027\uff09<\/td>\u9ad8\uff08RNA \u5b9a\u91cf\uff09<\/td>\u6975\u9ad8\uff08\u7a00\u6709\u76ee\u6a19\u6aa2\u6e2c\uff09<\/td><\/tr>
                        \u64cd\u4f5c\u96e3\u6613\u5ea6 Ease of Use<\/strong><\/td>\u7c21\u55ae<\/td>\u4e2d\u7b49\u8907\u96dc\u5ea6\uff08\u9700\u87a2\u5149\u63a2\u91dd\uff09<\/td>\u9700\u53cd\u8f49\u9304\u6b65\u9a5f<\/td>\u8f03\u8907\u96dc\uff08\u7d50\u5408 RT \u8207\u5373\u6642\u5b9a\u91cf\uff09<\/td>\u8907\u96dc\uff08\u9700\u6a23\u672c\u5206\u9694\uff09<\/td><\/tr>
                        \u6210\u672c Cost<\/strong><\/td>\u4f4e<\/td>\u4e2d\u81f3\u9ad8<\/td>\u4e2d\u7b49<\/td>\u9ad8<\/td>\u6975\u9ad8<\/td><\/tr>
                        \u6a23\u672c\u8655\u7406\u5bb9\u91cf Sample Volume Capacity<\/strong><\/td>\u4e2d\u7b49<\/td>\u5927<\/td>\u4e2d\u7b49<\/td>\u5927<\/td>\u5c0f<\/td><\/tr>
                        \u61c9\u7528 Uses<\/strong><\/td>\u57fa\u56e0\u8907\u88fd\u3001\u57fa\u56e0\u5206\u578b\u3001\u7a81\u8b8a\u6aa2\u6e2c<\/td>\u57fa\u56e0\u8868\u73fe\u5206\u6790\u3001\u75c5\u539f\u9ad4\u6aa2\u6e2c<\/td>RNA \u75c5\u6bd2\u6aa2\u6e2c\u3001\u57fa\u56e0\u8868\u73fe\u7814\u7a76<\/td>\u75c5\u6bd2\u8f09\u91cf\u76e3\u6e2c\u3001RNA \u5b9a\u91cf<\/td>\u7a00\u6709\u7a81\u8b8a\u6aa2\u6e2c\u3001\u7d55\u5c0d\u5b9a\u91cf<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

                        \u5176\u4ed6 DNA \u64f4\u589e\u6280\u8853<\/strong><\/h2>\n\n\n\n

                        \u96d6\u7136 PCR \u662f DNA \u64f4\u589e\u7684\u57fa\u77f3\uff0c\u4f46\u5176\u4ed6\u6280\u8853\u5982 \u6046\u6eab\u64f4\u589e\uff08isothermal amplification\uff09<\/strong>\u3001\u5168\u57fa\u56e0\u9ad4\u64f4\u589e\uff08whole genome amplification, WGA\uff09<\/strong> \u8207 \u6a4b\u5f0f\u64f4\u589e\uff08bridge amplification\uff09<\/strong> \u4e5f\u5404\u81ea\u5177\u5099\u7368\u7279\u512a\u52e2\u3002\u4ee5\u4e0b\u5c07\u6bd4\u8f03\u9019\u4e9b\u65b9\u6cd5\u7684\u512a\u7f3a\u9ede\uff0c\u4e26\u8207 PCR \u9032\u884c\u5c0d\u7167\u3002<\/p>\n\n\n\n

                        \u6046\u6eab\u64f4\u589e\uff08Isothermal Amplification\uff09<\/strong><\/h3>\n\n\n\n

                        \u6046\u6eab\u64f4\u589e\u662f\u5728 \u56fa\u5b9a\u6eab\u5ea6\u4e0b<\/strong> \u9032\u884c DNA \u64f4\u589e\uff0c\u4e0d\u540c\u65bc PCR \u9700\u8981\u5728\u4e0d\u540c\u6eab\u5ea6\u4e4b\u9593\u5faa\u74b0\u3002<\/p>\n\n\n\n

                        \u5e38\u898b\u6280\u8853\u5982 \u74b0\u4ecb\u5c0e\u7b49\u6eab\u64f4\u589e\uff08LAMP\uff09<\/strong> \u5c6c\u65bc\u6b64\u985e\u3002\u9019\u4e9b\u65b9\u6cd5\u66f4\u5feb\u901f\u4e14\u901a\u5e38\u66f4\u4fbf\u651c\uff0c\u56e0\u70ba\u4e0d\u9700\u8981\u71b1\u5faa\u74b0\u5100\u3002\u4f8b\u5982 LAMP \u53ef\u5728\u4e00\u5c0f\u6642\u5167\u5b8c\u6210 DNA \u64f4\u589e\uff0c\u7279\u5225\u9069\u5408\u7528\u65bc\u504f\u9060\u5730\u5340\u7684\u75be\u75c5\u8a3a\u65b7\u7b49\u73fe\u5834\u61c9\u7528\u3002<\/p>\n\n\n\n

                        \u7136\u800c\uff0c\u6046\u6eab\u64f4\u589e\u7684\u7279\u7570\u6027\u4f4e\u65bc PCR\uff0c\u53ef\u80fd\u5c0e\u81f4\u5047\u967d\u6027\u3002\u96d6\u7136\u7b49\u6eab\u65b9\u6cd5\u4ee5\u901f\u5ea6\u8207\u7c21\u4fbf\u898b\u9577\uff0c\u4f46 PCR \u5728\u7cbe\u6e96\u5ea6\u8207\u64f4\u589e\u7279\u5b9a DNA \u5e8f\u5217\u7684\u9748\u6d3b\u6027\u4e0a\u4ecd\u66f4\u5177\u512a\u52e2\uff0c\u7279\u5225\u662f\u5728\u9700\u8981\u8a73\u7d30\u5be6\u9a57\u5ba4\u5206\u6790\u7684\u5834\u5408\u3002<\/p>\n\n\n\n

                        \u5168\u57fa\u56e0\u9ad4\u64f4\u589e\uff08Whole Genome Amplification, WGA\uff09<\/strong><\/h3>\n\n\n\n

                        WGA \u7684\u76ee\u7684\u662f \u64f4\u589e\u6574\u500b\u57fa\u56e0\u9ad4<\/strong>\uff0c\u800c\u975e\u50c5\u9650\u65bc\u7279\u5b9a DNA \u5340\u6bb5\u3002<\/p>\n\n\n\n

                        \u9019\u9805\u6280\u8853\u5728\u8655\u7406\u975e\u5e38\u5fae\u91cf\u6216\u964d\u89e3\u7684 DNA \u6a23\u672c\u6642\u6975\u70ba\u6709\u7528\uff0c\u4f8b\u5982\u53e4\u4ee3\u6587\u7269\u6216\u55ae\u4e00\u7d30\u80de\u7684 DNA\u3002\u96d6\u7136 WGA \u80fd\u56de\u6536\u5927\u91cf\u907a\u50b3\u7269\u8cea\uff0c\u4f46\u53ef\u80fd\u7522\u751f\u504f\u5dee\uff0c\u5c0e\u81f4\u67d0\u4e9b DNA \u5340\u6bb5\u88ab\u904e\u5ea6\u4ee3\u8868\u3002<\/p>\n\n\n\n

                        \u76f8\u6bd4\u4e4b\u4e0b\uff0cPCR \u805a\u7126\u65bc\u7279\u5b9a\u5e8f\u5217\u7684\u64f4\u589e\uff0c\u5177\u5099\u9ad8\u4fdd\u771f\u5ea6\uff0c\u66f4\u9069\u5408\u7cbe\u6e96\u7684\u76ee\u6a19\u7814\u7a76\u3002WGA \u9069\u5408\u9700\u8981\u5ee3\u6cdb\u57fa\u56e0\u8cc7\u8a0a\u7684\u61c9\u7528\uff0c\u800c PCR \u5247\u5728\u7cbe\u78ba\u5ea6\u8207\u76ee\u6a19\u6027\u7814\u7a76\u4e0a\u66f4\u5177\u512a\u52e2\u3002<\/p>\n\n\n\n

                        \u6a4b\u5f0f\u64f4\u589e\uff08Bridge Amplification\uff09<\/strong><\/h3>\n\n\n\n

                        \u6a4b\u5f0f\u64f4\u589e\u662f\u4e00\u7a2e\u61c9\u7528\u65bc \u6b21\u4e16\u4ee3\u5b9a\u5e8f\uff08NGS\uff09<\/strong> \u7684\u65b9\u6cd5\u3002\u5176\u904e\u7a0b\u662f\u8b93\u55ae\u80a1 DNA \u8207\u8868\u9762\u4e0a\u7684\u63a5\u982d\u5e8f\u5217\uff08adapters\uff09\u7d50\u5408\uff0c\u5f62\u6210\u300c\u6a4b\u72c0\u7d50\u69cb\u300d\uff0c\u9032\u800c\u751f\u6210 DNA \u805a\u843d\uff0c\u7522\u751f\u6578\u767e\u842c\u4efd\u76f8\u540c DNA \u62f7\u8c9d\u4ee5\u4f9b\u5b9a\u5e8f\u4f7f\u7528\u3002<\/p>\n\n\n\n

                        \u9019\u7a2e\u65b9\u6cd5\u5c0d\u65bc\u5b9a\u5e8f\u6d41\u7a0b\u6975\u70ba\u9ad8\u6548\uff0c\u4f46\u4e0d\u9069\u7528\u65bc\u5176\u4ed6 DNA \u5206\u6790\u3002\u76f8\u6bd4\u4e4b\u4e0b\uff0cPCR \u53ef\u9748\u6d3b\u61c9\u7528\u65bc\u5404\u7a2e\u5834\u666f\uff08\u5982\u8a3a\u65b7\u8207\u57fa\u56e0\u8907\u88fd\uff09\uff0c\u800c\u6a4b\u5f0f\u64f4\u589e\u50c5\u9650\u65bc NGS\u3002\u96d6\u7136\u6a4b\u5f0f\u64f4\u589e\u78ba\u4fdd\u4e86\u9ad8\u901a\u91cf\u5b9a\u5e8f\uff0c\u4f46 PCR \u4ecd\u56e0\u5176\u5ee3\u6cdb\u61c9\u7528\u6027\u8207\u5728\u591a\u6578\u5206\u5b50\u751f\u7269\u5b78\u5be6\u9a57\u5ba4\u7684\u666e\u53ca\u6027\u800c\u5c45\u65bc\u6838\u5fc3\u5730\u4f4d\u3002<\/p>\n\n\n\n

                        PCR \u7684\u66ff\u4ee3\u65b9\u6848\uff1aMolsentech \u7684\u7121\u64f4\u589e\u6280\u8853<\/strong><\/h2>\n\n\n\n

                        PCR \u8207\u5176\u9032\u968e\u8b8a\u9ad4\u96d6\u7136\u5fb9\u5e95\u9769\u65b0\u4e86\u5206\u5b50\u751f\u7269\u5b78\uff0c\u4f7f\u6838\u9178\u64f4\u589e\u8207\u5206\u6790\u6210\u70ba\u53ef\u80fd\uff0c\u4f46\u5176\u6d41\u7a0b\u5f80\u5f80\u8907\u96dc\uff0c\u9700\u6602\u8cb4\u8a66\u5291\u8207\u5c08\u7528\u8a2d\u5099\u3002\u5118\u7ba1\u64f4\u589e\u529f\u80fd\u5f37\u5927\uff0c\u4f46\u4e5f\u589e\u52a0\u4e86\u8a3a\u65b7\u904e\u7a0b\u7684\u8907\u96dc\u5ea6\u8207\u8017\u6642\u3002<\/p>\n\n\n\n

                        Molsentech \u6b63\u4ee5\u5176 Bio-FET \u6676\u7247\u6280\u8853<\/strong> \u6253\u7834\u9019\u4e9b\u9650\u5236\uff0c\u5be6\u73fe \u7121\u9700\u64f4\u589e\u7684\u6aa2\u6e2c<\/strong>\u3002<\/p>\n\n\n\n

                        \u4f5c\u70ba\u53f0\u7063\u7684\u524d\u77bb\u751f\u6280\u65b0\u5275\uff0cMolsentech \u6b63\u4ee5\u5148\u9032\u7684 \u5948\u7c73\u6280\u8853\u3001\u5316\u5b78\u4fee\u98fe\u3001AI \u6f14\u7b97\u6cd5\u8207\u81ea\u52d5\u5316\u7cfb\u7d71<\/strong>\uff0c\u6253\u9020\u8d85\u9ad8\u9748\u654f\u3001\u5feb\u901f\u4e14\u7cbe\u6e96\u7684\u751f\u7269\u5206\u5b50\u6aa2\u6e2c\u5e73\u53f0\uff0c\u5fb9\u5e95\u91cd\u5851\u8a3a\u65b7\u9818\u57df\u3002<\/p>\n\n\n\n

                        \u5728 \u65b0\u51a0\u75ab\u60c5<\/strong> \u671f\u9593\uff0cMolsentech \u7684\u6838\u9178\u6aa2\u6e2c\u6676\u7247\u7372\u5f97\u53f0\u7063 \u7dca\u6025\u4f7f\u7528\u6388\u6b0a\uff08EUA\uff09<\/strong>\uff0c\u4e26\u65bc\u81e8\u5e8a\u5be6\u969b\u61c9\u7528\u4e2d\u5c55\u73fe\u9ad8\u5ea6\u6548\u80fd\u3002\u9664\u65b0\u51a0\u4e4b\u5916\uff0c\u6b64\u5e73\u53f0\u66f4\u91dd\u5c0d \u963f\u8332\u6d77\u9ed8\u75c7\u3001\u764c\u75c7\u8207\u50b3\u67d3\u75c5<\/strong> \u7b49\u91cd\u5927\u75be\u75c5\uff0c\u5be6\u73fe\u66f4\u65e9\u671f\u8a3a\u65b7\u8207\u62ef\u6551\u751f\u547d\u7684\u81e8\u5e8a\u61c9\u7528\u3002\u6211\u5011\u6b63\u4ee5\u985b\u8986\u6027\u7684\u6280\u8853\u63a8\u52d5\u91ab\u7642\u9769\u65b0\u3001\u62ef\u6551\u751f\u547d\u3002\u7acb\u5373\u806f\u7e6b Molsentech<\/strong>\uff0c\u4e86\u89e3\u6211\u5011\u7684\u6280\u8853\u5982\u4f55\u5f15\u9818\u60a8\u9032\u5165\u672a\u4f86\u8a3a\u65b7\u7684\u5168\u65b0\u5883\u754c\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

                        \u805a\u5408\u9176\u93c8\u5f0f\u53cd\u61c9\uff08Polymerase Chain Reaction, PCR\uff09\u662f\u4e00\u7a2e\u57fa\u790e\u7684\u5be6\u9a57\u5ba4\u6280\u8853\uff0c\u7528\u65bc\u64f4\u589e […]<\/p>\n","protected":false},"featured_media":1538,"template":"","meta":{"_acf_changed":false},"class_list":["post-4856","blog_post","type-blog_post","status-publish","has-post-thumbnail","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/molsentech.iapplabz.co.in\/zh-hant\/wp-json\/wp\/v2\/blog_post\/4856","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/molsentech.iapplabz.co.in\/zh-hant\/wp-json\/wp\/v2\/blog_post"}],"about":[{"href":"https:\/\/molsentech.iapplabz.co.in\/zh-hant\/wp-json\/wp\/v2\/types\/blog_post"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/molsentech.iapplabz.co.in\/zh-hant\/wp-json\/wp\/v2\/media\/1538"}],"wp:attachment":[{"href":"https:\/\/molsentech.iapplabz.co.in\/zh-hant\/wp-json\/wp\/v2\/media?parent=4856"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}